{"id":716,"date":"2020-10-22T03:09:44","date_gmt":"2020-10-22T03:09:44","guid":{"rendered":"http:\/\/cvsc.upcebu.edu.ph\/?p=716"},"modified":"2025-11-13T10:02:56","modified_gmt":"2025-11-13T10:02:56","slug":"discovery-of-new-antibacterial-accramycins-from-a-genetic-variant-of-the-soil-bacterium-streptomyces-sp-ma37","status":"publish","type":"project","link":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/project\/discovery-of-new-antibacterial-accramycins-from-a-genetic-variant-of-the-soil-bacterium-streptomyces-sp-ma37\/","title":{"rendered":"Discovery of New Antibacterial Accramycins from a Genetic Variant of the Soil Bacterium, Streptomyces sp. MA37"},"content":{"rendered":"\n

Fleurdeliz Maglangit<\/strong><\/p>\n\n\n\n

Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK; y.zhang7.18@abdn.ac.uk<\/p>\n\n\n\n

Department of Biology and Environmental Science, College of Science, University of the Philippines Cebu, Lahug, Cebu City 6000, Philippines<\/p>\n\n\n\n

Yuting Zhang<\/strong><\/p>\n\n\n\n

Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK; y.zhang7.18@abdn.ac.uk<\/p>\n\n\n\n

Kwaku Kyeremeh<\/strong><\/p>\n\n\n\n

Department of Chemistry, University of Ghana, Legon\u2010Accra P.O. Box LG56, Ghana; kkyeremeh@ug.edu.gh<\/p>\n\n\n\n

Hai Deng<\/strong><\/p>\n\n\n\n

Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK; y.zhang7.18@abdn.ac.uk<\/p>\n\n\n\n

Correspondence: ffmaglangit@up.edu.ph (F.M.); h.deng@abdn.ac.uk (H.D.); Tel.: +63\u201032\u2010232\u20108185 (F.M.); Tel.: +44\u20101224\u2010272\u2010953 (H.D.); Fax: +63\u201032\u2010232\u20108185 (F.M.); Fax: +44\u20101224\u201027\u20102291 (H.D.)<\/p>\n\n\n\n

\"\"<\/figure>\n\n\n\n

Abstract<\/strong><\/p>\n\n\n\n

Continued mining of natural products from the strain Streptomyces sp. MA37 in our laboratory led to the discovery of a minor specialized metabolite (SM) called accramycin A. Owing to its low yield (0.2 mg\/L) in the wild type strain, we investigated the roles of regulatory genes in the corresponding biosynthetic gene cluster (acc BGC) through gene inactivation with the aim of improving the titer of this compound. One of the resulting mutants (\u0394accJ) dramatically upregulated the production of accramycin A 1 by 330\u2010fold (66 mg\/L). Furthermore, ten new metabolites, accramycins B\u2013K 2\u201311, were discovered, together with two known compounds, naphthacemycin B1 12 and fasamycin C 13 from the mutant extract. This suggested that accJ<\/em>, annotated as multiple antibiotic resistance regulator (MarR), is a negative regulator gene in the accramycin biosynthesis. Compounds 1\u201313 inhibited the Gram\u2010positive pathogens (Staphylococcus aureus, Enterococcus faecalis<\/em>) and clinical isolates Enterococcus faecium<\/em> (K59\u201068 and K60\u201039) and Staphylococcus haemolyticus<\/em> with minimal inhibitory concentration (MIC) values in the range of 1.5\u201312.5 \u03bcg\/mL. Remarkably, compounds 1\u201313 displayed superior activity against K60\u201039 (MIC = 3.1\u20136.3 \u03bcg\/mL) compared to ampicillin (MIC = 25 \u03bcg\/mL), and offered promising potential for the development of accramycin based antibiotics that target multidrug\u2010resistant Enterococcus clinical isolates. Our results highlight the importance of identifying the roles of regulatory genes in natural product discovery.<\/p>\n\n\n\n


Keywords: <\/strong>accramycin; type II polyketides; Streptomyces sp. MA37; regulatory genes; gene inactivation; titer improvement; antibacterial activities; multidrug-resistant Enterococcus<\/p>\n","protected":false},"excerpt":{"rendered":"

Fleurdeliz Maglangit Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK; y.zhang7.18@abdn.ac.uk Department of Biology and Environmental Science, College of Science, University of the Philippines Cebu, Lahug, Cebu City 6000, Philippines Yuting Zhang Department of Chemistry, University of Aberdeen, Aberdeen AB24 3UE, Scotland, UK; y.zhang7.18@abdn.ac.uk Kwaku Kyeremeh Department of Chemistry, University of Ghana, […]<\/p>\n","protected":false},"author":1,"featured_media":0,"template":"","meta":{"_et_pb_use_builder":"","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"project_category":[],"project_tag":[],"class_list":["post-716","project","type-project","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/716","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project"}],"about":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/types\/project"}],"author":[{"embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":1,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/716\/revisions"}],"predecessor-version":[{"id":2910,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/716\/revisions\/2910"}],"wp:attachment":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/media?parent=716"}],"wp:term":[{"taxonomy":"project_category","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project_category?post=716"},{"taxonomy":"project_tag","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project_tag?post=716"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}