{"id":1548,"date":"2024-12-10T05:19:23","date_gmt":"2024-12-10T05:19:23","guid":{"rendered":"https:\/\/cvsc.upcebu.edu.ph\/?p=1548"},"modified":"2025-11-12T14:40:10","modified_gmt":"2025-11-12T14:40:10","slug":"characterization-of-the-baeyer-villiger-monooxygenase-in-the-pathway-of-the-bacterial-pyrrolizidine-alkaloids-legonmycins","status":"publish","type":"project","link":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/project\/characterization-of-the-baeyer-villiger-monooxygenase-in-the-pathway-of-the-bacterial-pyrrolizidine-alkaloids-legonmycins\/","title":{"rendered":"Characterization of the Baeyer\u2013Villiger monooxygenase in the pathway of the bacterial pyrrolizidine alkaloids, legonmycins"},"content":{"rendered":"

[et_pb_section fb_built=”1″ theme_builder_area=”post_content” _builder_version=”4.26.0″ _module_preset=”default”][et_pb_row _builder_version=”4.26.0″ _module_preset=”default” column_structure=”1_2,1_2″ theme_builder_area=”post_content”][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”1_2″ theme_builder_area=”post_content”][et_pb_image src=”http:\/\/172.17.1.35\/wp-content\/uploads\/2024\/12\/Mglangit-RSC.png” _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” title_text=”Mglangit-RSC” box_shadow_style=”preset4″ box_shadow_blur=”6px” hover_enabled=”0″ sticky_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”1_2″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

Authors<\/strong><\/h2>\n

Shan Wang<\/a><\/p>\n

<\/span>Fleurdeliz Maglangit<\/a><\/span><\/strong><\/span>
Department of Biology and Environmental Science, University of the Philippines, Cebu<\/h4>\n

<\/span>Qing Fang<\/a><\/span><\/p>\n

Kwaku Kyeremeh<\/a><\/p>\n

<\/span>Hai Deng<\/a><\/span><\/p>\n

 <\/p>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content”][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”4_4″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

Abstract<\/strong><\/h3>\n
\n
\n

The Baeyer\u2013Villiger monooxygenase (BVMO), LgnC, plays a crucial role in the biosynthesis of bacterial pyrrolizidine alkaloids, legonmycins. It processes bicyclic indolizidine substrates generated from the coordinative action of two non-ribosomal peptide synthetases (LgnB and LgnD) and the standalone type II thioesterase-like enzyme (LgnA). It has been demonstrated that the enzyme selectively inserts molecular oxygen into the carbon\u2013carbon bond adjacent to the carbonyl group in legonindolizidines to form bicyclic 1,3-oxazepine carbamate intermediates. After ring opening and contraction, the most advanced products, prelegonmycins, are formed. However, factors controlling the final hydroxylation step and how the enzyme handles the substrates have remained elusive. In this study, we show that the final hydroxylation at the activated carbon of the electron-rich pyrrole system is attributed to either spontaneous oxidation or the action of an endogenous redox reagent. Substrate docking on the structural model of LgnC combined with site-directed mutagenesis allows the identification of several key amino acids that are essential for substrate\/intermediate binding and a mechanism of LgnC-catalysed transformation is proposed.<\/p>\n<\/div>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.26.0″ _module_preset=”default” column_structure=”1_2,1_2″ theme_builder_area=”post_content” box_shadow_style=”preset1″ hover_enabled=”0″ sticky_enabled=”0″][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”1_2″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

Link to the article:<\/strong><\/h3>\n

https:\/\/doi.org\/10.1039\/D4CB00186A<\/a>\u00a0<\/p>\n

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Authors Shan Wang Fleurdeliz MaglangitDepartment of Biology and Environmental Science, University of the Philippines, Cebu Qing Fang Kwaku Kyeremeh Hai Deng   Abstract The Baeyer\u2013Villiger monooxygenase (BVMO), LgnC, plays a crucial role in the biosynthesis of bacterial pyrrolizidine alkaloids, legonmycins. It processes bicyclic indolizidine substrates generated from the coordinative action of two non-ribosomal peptide synthetases […]<\/p>\n","protected":false},"author":1,"featured_media":0,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"project_category":[34],"project_tag":[],"class_list":["post-1548","project","type-project","status-publish","hentry","project_category-recent-publications"],"_links":{"self":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/1548","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project"}],"about":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/types\/project"}],"author":[{"embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":1,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/1548\/revisions"}],"predecessor-version":[{"id":1888,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/1548\/revisions\/1888"}],"wp:attachment":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/media?parent=1548"}],"wp:term":[{"taxonomy":"project_category","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project_category?post=1548"},{"taxonomy":"project_tag","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project_tag?post=1548"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}