{"id":1488,"date":"2024-09-03T09:51:23","date_gmt":"2024-09-03T09:51:23","guid":{"rendered":"https:\/\/cvsc.upcebu.edu.ph\/?p=1488"},"modified":"2025-11-12T14:41:06","modified_gmt":"2025-11-12T14:41:06","slug":"analysis-of-cenkr-essentiality-in-sinorhizobium-meliloti-and-its-activity-at-a-target-gene-promoter-in-vivo","status":"publish","type":"project","link":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/project\/analysis-of-cenkr-essentiality-in-sinorhizobium-meliloti-and-its-activity-at-a-target-gene-promoter-in-vivo\/","title":{"rendered":"Analysis of CenKR essentiality in\u00a0Sinorhizobium meliloti\u00a0and its activity at a target gene promoter\u00a0in vivo"},"content":{"rendered":"

[et_pb_section fb_built=”1″ theme_builder_area=”post_content” _builder_version=”4.26.0″ _module_preset=”default”][et_pb_row _builder_version=”4.26.0″ _module_preset=”default” column_structure=”3_5,2_5″ theme_builder_area=”post_content”][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”3_5″ theme_builder_area=”post_content”][et_pb_image src=”http:\/\/172.17.1.35\/wp-content\/uploads\/2024\/10\/Analysis-of-CenKR-Bensig.jpeg” _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” title_text=”Analysis of CenKR-Bensig” box_shadow_style=”preset2″ box_shadow_horizontal=”27px” box_shadow_vertical=”-36px” box_shadow_blur=”10px” hover_enabled=”0″ sticky_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”2_5″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

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Authors<\/strong><\/h2>\n

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Isabela R Freire<\/strong><\/p>\n

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Eukene O Bensig<\/strong><\/span><\/h3>\n<\/div>\n
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Department of Biology and Environmental Science, University of the Philippines Cebu<\/div>\n
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ZiYu Kuang<\/span><\/strong><\/div>\n
Shawn R MacLellan<\/span><\/span><\/strong><\/div>\n<\/div>\n<\/div>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content”][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”4_4″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

Abstract<\/strong><\/h2>\n

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The two-component regulatory system CenK\u2013CenR has recently emerged as a regulator of cell envelope and cell division processes in the alpha-proteobacteria. In\u00a0<\/span>Sinorhizobium meliloti<\/em>, CenK\u2013CenR regulates the expression of SrlA, a thioredoxin-domain protein of unknown function. Deletion of\u00a0<\/span>srlA<\/em>\u00a0<\/span>causes sensitivity to salt and oxidizing agents on solid growth medium. In this work, we report that the response regulator CenR, but not the histidine kinase CenK, is essential for cell viability in\u00a0<\/span>S. meliloti<\/em>. We also demonstrate that phosphorylation of the target residue D55 is not required for viability, suggesting that the unphosphorylated transcription factor sufficiently regulates expression of one or more essential genes in the genome. Using transcription assays and phenotype testing we examine CenK\u2013CenR-dependent activation of the\u00a0<\/span>srlA<\/em>\u00a0<\/span>promoter and demonstrate its absolute dependence on phosphoryl-CenR for activity and that the CenR substitution D55E acts as a phosphomimetic that partially restores activity at the\u00a0<\/span>srlA<\/em>\u00a0<\/span>promoter in the absence of phosphorylation by CenK. Finally, we report a mutational analysis of the CenR binding site in the\u00a0<\/span>srlA<\/em>\u00a0<\/span>promoter required for transcriptional activation.<\/p>\n<\/section>\n

[\/et_pb_text][\/et_pb_column][\/et_pb_row][et_pb_row _builder_version=”4.26.0″ _module_preset=”default” column_structure=”1_2,1_2″ theme_builder_area=”post_content” box_shadow_style=”preset1″ hover_enabled=”0″ sticky_enabled=”0″][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”1_2″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

Keywords:<\/strong><\/h2>\n

rhizobia<\/a>,\u00a0<\/span>two-component<\/a>,\u00a0<\/span>promoter<\/a>,\u00a0<\/span>transcription regulation<\/a>,\u00a0<\/span>thioredoxin<\/a><\/p>\n

[\/et_pb_text][\/et_pb_column][et_pb_column _builder_version=”4.26.0″ _module_preset=”default” type=”1_2″ theme_builder_area=”post_content”][et_pb_text _builder_version=”4.26.0″ _module_preset=”default” theme_builder_area=”post_content” hover_enabled=”0″ sticky_enabled=”0″]<\/p>\n

Link to the article:<\/strong><\/h2>\n

https:\/\/doi.org\/10.1093\/femsle\/fnae061<\/a>\u00a0<\/p>\n

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Authors Isabela R Freire Eukene O Bensig Department of Biology and Environmental Science, University of the Philippines Cebu ZiYu Kuang Shawn R MacLellan Abstract The two-component regulatory system CenK\u2013CenR has recently emerged as a regulator of cell envelope and cell division processes in the alpha-proteobacteria. In\u00a0Sinorhizobium meliloti, CenK\u2013CenR regulates the expression of SrlA, a thioredoxin-domain […]<\/p>\n","protected":false},"author":1,"featured_media":0,"template":"","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"project_category":[34],"project_tag":[],"class_list":["post-1488","project","type-project","status-publish","hentry","project_category-recent-publications"],"_links":{"self":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/1488","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project"}],"about":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/types\/project"}],"author":[{"embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/users\/1"}],"version-history":[{"count":1,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/1488\/revisions"}],"predecessor-version":[{"id":1901,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project\/1488\/revisions\/1901"}],"wp:attachment":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/media?parent=1488"}],"wp:term":[{"taxonomy":"project_category","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project_category?post=1488"},{"taxonomy":"project_tag","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/project_tag?post=1488"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}