[et_pb_section fb_built=”1″ theme_builder_area=”post_content” _builder_version=”4.27.7″ _module_preset=”default”][et_pb_row column_structure=”1_3,2_3″ _builder_version=”4.27.4″ _module_preset=”default” custom_padding=”40px|||||” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_column type=”1_3″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_image src=”http:\/\/cvsc.upcebu.edu.ph\/wp-content\/uploads\/2026\/10\/Badon-EMM.png” title_text=”Badon-EMM” _builder_version=”4.27.7″ _module_preset=”default” hover_enabled=”0″ box_shadow_style=”preset2″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″][\/et_pb_image][\/et_pb_column][et_pb_column type=”2_3″ _builder_version=”4.27.4″ _module_preset=”default” global_colors_info=”{}” theme_builder_area=”post_content”][et_pb_text _builder_version=”4.27.7″ _module_preset=”default” text_font_size=”15px” text_line_height=”1.2em” custom_padding=”0px|||||” hover_enabled=”0″ global_colors_info=”{}” theme_builder_area=”post_content” sticky_enabled=”0″]<\/p>\n
Lead Researcher(s): <\/strong>Lee Wha Gwon, Isabel Wen Badon<\/strong>, Youngjeon Lee, Ho Joong Kim, and Seung Hwan Lee<\/p>\n Status:<\/strong> Published<\/p>\n Abstract\/summary:<\/strong> In recent years, DNA engineering technology has undergone significant advancements, with clustered regularly interspaced short palindromic repeats (CRISPR)-based target-specific DNA insertion emerging as one of the most rapidly expanding and widely studied approaches. Traditional DNA insertion technologies employing recombinases typically involve introducing foreign DNA into genes in vivo by either pre-engineering recognition sequences specific to the recombinase or through genetic crossing to incorporate the requisite recognition sequence into the target gene. However, CRISPR-based gene insertion technologies have advanced to streamline this engineering process by combining the CRISPR\u2013Cas module with recombinase enzymes. This process enables accurate and efficient one-step insertion of foreign DNA into the target gene in vivo. Here we provide an overview of the latest developments in CRISPR-based gene insertion technologies and discusses their potential future applications.<\/span><\/span><\/p>\n [\/et_pb_text][et_pb_button button_text=”Downloadable PDF” _builder_version=”4.27.7″ _module_preset=”default” theme_builder_area=”post_content” url_new_window=”on” button_url=”https:\/\/www.nature.com\/articles\/s12276-025-01530-0″ custom_button=”on” button_text_color=”#8A1538″ hover_enabled=”0″ sticky_enabled=”0″][\/et_pb_button][\/et_pb_column][\/et_pb_row][\/et_pb_section]<\/p>\n","protected":false},"excerpt":{"rendered":" Lead Researcher(s): Lee Wha Gwon, Isabel Wen Badon, Youngjeon Lee, Ho Joong Kim, and Seung Hwan LeeStatus: Published Abstract\/summary: In recent years, DNA engineering technology has undergone significant advancements, with clustered regularly interspaced short palindromic repeats (CRISPR)-based target-specific DNA insertion emerging as one of the most rapidly expanding and widely studied approaches. Traditional DNA insertion […]<\/p>\n","protected":false},"author":7,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"_et_pb_use_builder":"on","_et_pb_old_content":"","_et_gb_content_width":"","footnotes":""},"categories":[96,138,7,117,111],"tags":[],"class_list":["post-3709","post","type-post","status-publish","format-standard","hentry","category-cos-research-reports","category-journal-article","category-publications","category-sdg-3","category-sdg-9"],"_links":{"self":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/posts\/3709","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/users\/7"}],"replies":[{"embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/comments?post=3709"}],"version-history":[{"count":2,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/posts\/3709\/revisions"}],"predecessor-version":[{"id":3713,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/posts\/3709\/revisions\/3713"}],"wp:attachment":[{"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/media?parent=3709"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/categories?post=3709"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/cvsc.upcebu.edu.ph\/index.php\/wp-json\/wp\/v2\/tags?post=3709"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}